BAIT

TCP2

F28J12.50, F28J12_50, TEOSINTE BRANCHED 1, cycloidea and PCF transcription factor 2, AT4G18390
transcription factor TCP2
GO Process (5)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

PRR5

APRR5, T31K7.5, T31K7_5, pseudo-response regulator 5, AT5G24470
pseudo-response regulator 5
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping.

Trigg SA, Garza RM, MacWilliams A, Nery JR, Bartlett A, Castanon R, Goubil A, Feeney J, O'Malley R, Huang SC, Zhang ZZ, Galli M, Ecker JR

Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase interaction reporter to intracellularly fuse the coding sequences of two interacting proteins and next-generation DNA sequencing to identify these interactions en masse. We applied CrY2H-seq to investigate ... [more]

Nat. Methods Aug. 01, 2017; 14(8);819-825 [Pubmed: 28650476]

Throughput

  • High Throughput

Additional Notes

  • CrY2H-seq (Cre reporter-mediated yeast two-hybrid coupled with next-generation sequencing)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TCP2 PRR5
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By