BAIT
GRF9
AtGRF9, F4L23.1, F4L23_1, growth-regulating factor 9, AT2G45480
growth-regulating factor 9
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Arabidopsis thaliana (Columbia)
PREY
GIF3
A_TM018A10.22, A_TM018A10_22, GRF1-interacting factor 3, T18A10.15, T18A10_15, AT4G00850
GRF1-interacting factor 3
GO Process (2)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping.
Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase interaction reporter to intracellularly fuse the coding sequences of two interacting proteins and next-generation DNA sequencing to identify these interactions en masse. We applied CrY2H-seq to investigate ... [more]
Nat. Methods Aug. 01, 2017; 14(8);819-825 [Pubmed: 28650476]
Throughput
- High Throughput
Additional Notes
- CrY2H-seq (Cre reporter-mediated yeast two-hybrid coupled with next-generation sequencing)