STH2
Gene Ontology Biological Process
Gene Ontology Molecular Function
HY5
Gene Ontology Biological Process
- gibberellic acid mediated signaling pathway [IMP]
- photomorphogenesis [TAS]
- positive regulation of anthocyanin metabolic process [IMP]
- positive regulation of circadian rhythm [IMP]
- red or far-red light signaling pathway [IMP]
- regulation of photomorphogenesis [IMP]
- regulation of transcription, DNA-templated [ISS, TAS]
- response to UV-B [IEP, IGI, IMP]
- response to abscisic acid [IMP]
- response to far red light [IEP]
- response to karrikin [IEP]
- response to red light [IEP]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping.
Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase interaction reporter to intracellularly fuse the coding sequences of two interacting proteins and next-generation DNA sequencing to identify these interactions en masse. We applied CrY2H-seq to investigate ... [more]
Throughput
- High Throughput
Additional Notes
- CrY2H-seq (Cre reporter-mediated yeast two-hybrid coupled with next-generation sequencing)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STH2 HY5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STH2 HY5 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | - | |
| STH2 HY5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| HY5 STH2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |