BAIT

PTF1

CYCLOIDEA AND PCF TRANSCRIPTION FACTOR 13, F1C9.6, TCP13, TEOSINTE BRANCHED1, TFPD, plastid transcription factor 1, AT3G02150
transcription factor TCP13
Arabidopsis thaliana (Columbia)
PREY

SPL

F27G19.11, NOZZLE, NZZ, SPOROCYTELESS, AT4G27330
putative transcription factor SPL
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping.

Trigg SA, Garza RM, MacWilliams A, Nery JR, Bartlett A, Castanon R, Goubil A, Feeney J, O'Malley R, Huang SC, Zhang ZZ, Galli M, Ecker JR

Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase interaction reporter to intracellularly fuse the coding sequences of two interacting proteins and next-generation DNA sequencing to identify these interactions en masse. We applied CrY2H-seq to investigate ... [more]

Nat. Methods Aug. 01, 2017; 14(8);819-825 [Pubmed: 28650476]

Throughput

  • High Throughput

Additional Notes

  • CrY2H-seq (Cre reporter-mediated yeast two-hybrid coupled with next-generation sequencing)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPL PTF1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By