BAIT
GRF6
AtGRF6, F5K7.4, F5K7_4, growth-regulating factor 6, AT2G06200
growth-regulating factor 6
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Arabidopsis thaliana (Columbia)
PREY
AN3
ANGUSTIFOLIA 3, ARABIDOPSIS GRF1-INTERACTING FACTOR 1, ATGIF1, F4I4.20, F4I4_20, GIF, GIF1, GRF1-INTERACTING FACTOR, GRF1-INTERACTING FACTOR 1, AT5G28640
GRF1-interacting factor 1
GO Process (3)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping.
Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase interaction reporter to intracellularly fuse the coding sequences of two interacting proteins and next-generation DNA sequencing to identify these interactions en masse. We applied CrY2H-seq to investigate ... [more]
Nat. Methods Aug. 01, 2017; 14(8);819-825 [Pubmed: 28650476]
Throughput
- High Throughput
Additional Notes
- CrY2H-seq (Cre reporter-mediated yeast two-hybrid coupled with next-generation sequencing)