Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.

MacDonald E, Brown L, Selvais A, Liu H, Waring T, Newman D, Bithell J, Grimes D, Urbe S, Clague MJ, Zech T

Transmembrane proteins in the sorting endosome are either recycled to their point of origin or destined for lysosomal degradation. Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In this study, we uncover an alternative role for the ESCRT-0 component hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) in promoting ... [more]

J. Cell Biol. Jul. 02, 2018; 217(7);2549-2564 [Pubmed: 29891722]

Throughput

  • Low Throughput

Additional Notes

  • interaction identified based on both standard co-localization as well as PLA (proximity ligation assay)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
WASH1 HGS
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2742409

Curated By

  • BioGRID