Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and characterization of the BRI2 interactome in the brain.

Martins F, Marafona AM, Pereira CD, Mueller T, Loosse C, Kolbe K, da Cruz E Silva OAB, Rebelo S

BRI family proteins are ubiquitous type II transmembrane proteins but BRI2 is highly expressed in some neuronal tissues. Possible BRI2 functions include neuronal maturation and differentiation. Protein complexes appear to be important in mediating its functions. Previously described BRI2 interactors include the Alzheimer's amyloid precursor protein and protein phosphatase 1, but clearly the identification of novel interactors provides an important ... [more]

Sci Rep Feb. 23, 2018; 8(1);3548 [Pubmed: 29476059]

Throughput

  • High Throughput

Curated By

  • BioGRID