UBE2L3
Gene Ontology Biological Process
- cell cycle phase transition [IMP]
- cell proliferation [IMP]
- cellular protein modification process [TAS]
- cellular response to glucocorticoid stimulus [IDA]
- cellular response to steroid hormone stimulus [IMP]
- positive regulation of protein ubiquitination [IGI]
- positive regulation of ubiquitin-protein transferase activity [IGI]
- protein K11-linked ubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription, DNA-templated [IDA, IMP]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RNF216
Gene Ontology Biological Process
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.
Ubiquitination is initiated by transfer of ubiquitin (Ub) from a ubiquitin-activating enzyme (E1) to a ubiquitin-conjugating enzyme (E2), producing a covalently linked intermediate (E2-Ub) 1 . Ubiquitin ligases (E3s) of the 'really interesting new gene' (RING) class recruit E2-Ub via their RING domain and then mediate direct transfer of ubiquitin to substrates 2 . By contrast, 'homologous to E6-AP carboxy ... [more]
Throughput
- High Throughput
Additional Notes
- bait proteins from cell lysate were identified by mass spec in two separate experiments as proteins covalently attached via an aborted transthiolation attempt to a suicide substrate composed of both the E2s UBE2D2 and UBE2L3 attached in vitro to a tagged modified ubiquitin
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RNF216 UBE2L3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| UBE2L3 RNF216 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RNF216 UBE2L3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2527826 | |
| RNF216 UBE2L3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RNF216 UBE2L3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RNF216 UBE2L3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2694825 |
Curated By
- BioGRID