UBE2L3
Gene Ontology Biological Process
- cell cycle phase transition [IMP]
- cell proliferation [IMP]
- cellular protein modification process [TAS]
- cellular response to glucocorticoid stimulus [IDA]
- cellular response to steroid hormone stimulus [IMP]
- positive regulation of protein ubiquitination [IGI]
- positive regulation of ubiquitin-protein transferase activity [IGI]
- protein K11-linked ubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription, DNA-templated [IDA, IMP]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RNF14
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- positive regulation of transcription, DNA-templated [IDA, NAS]
- protein ubiquitination [IEP]
- regulation of androgen receptor signaling pathway [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription, DNA-templated [IDA]
- response to estradiol [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.
Ubiquitination is initiated by transfer of ubiquitin (Ub) from a ubiquitin-activating enzyme (E1) to a ubiquitin-conjugating enzyme (E2), producing a covalently linked intermediate (E2-Ub) 1 . Ubiquitin ligases (E3s) of the 'really interesting new gene' (RING) class recruit E2-Ub via their RING domain and then mediate direct transfer of ubiquitin to substrates 2 . By contrast, 'homologous to E6-AP carboxy ... [more]
Throughput
- High Throughput
Additional Notes
- bait proteins from cell lysate were identified by mass spec in two separate experiments as proteins covalently attached via an aborted transthiolation attempt to a suicide substrate composed of both the E2s UBE2D2 and UBE2L3 attached in vitro to a tagged modified ubiquitin
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2L3 RNF14 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| UBE2L3 RNF14 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| RNF14 UBE2L3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| RNF14 UBE2L3 | Surface Display Surface Display An interaction is inferred when a protein of interest is fused to a surface protein and assayed for interaction with another protein or ligand. The surface fusion is often expressed in yeast and termed yeast display (PUBMED: 39322662), but can also be expressed in bacteriophage and termed phage display (PUBMED: 10585717), or other microbes and termed bacterial surface display(PUBMED: 21146237). The displayed protein is then used to screen potential binding partners (such as antibodies, peptides, or other proteins). Binding can then be detected by the presence or cellular uptake (PUBMED: 39322662, figure 1c) of a fluorescent reporter. Ribosome display (PUBMED: 33514045) is also considered to be a type of surface display. | High | - | BioGRID | 3872309 |
Curated By
- BioGRID