UBE2L3
Gene Ontology Biological Process
- cell cycle phase transition [IMP]
- cell proliferation [IMP]
- cellular protein modification process [TAS]
- cellular response to glucocorticoid stimulus [IDA]
- cellular response to steroid hormone stimulus [IMP]
- positive regulation of protein ubiquitination [IGI]
- positive regulation of ubiquitin-protein transferase activity [IGI]
- protein K11-linked ubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription, DNA-templated [IDA, IMP]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TRIM25
Gene Ontology Biological Process
- innate immune response [IDA, TAS]
- negative regulation of type I interferon production [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- regulation of viral entry into host cell [IDA]
- regulation of viral release from host cell [IMP]
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.
Ubiquitination is initiated by transfer of ubiquitin (Ub) from a ubiquitin-activating enzyme (E1) to a ubiquitin-conjugating enzyme (E2), producing a covalently linked intermediate (E2-Ub) 1 . Ubiquitin ligases (E3s) of the 'really interesting new gene' (RING) class recruit E2-Ub via their RING domain and then mediate direct transfer of ubiquitin to substrates 2 . By contrast, 'homologous to E6-AP carboxy ... [more]
Throughput
- High Throughput
Additional Notes
- bait proteins from cell lysate were identified by mass spec in two separate experiments as proteins covalently attached via an aborted transthiolation attempt to a suicide substrate composed of both the E2s UBE2D2 and UBE2L3 attached in vitro to a tagged modified ubiquitin
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRIM25 UBE2L3 | Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis. | High | - | BioGRID | - |
Curated By
- BioGRID