BAIT
UBE2D2
E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B
ubiquitin-conjugating enzyme E2D 2
GO Process (13)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- cellular protein modification process [TAS]
- cellular response to hypoxia [TAS]
- innate immune response [TAS]
- protein K48-linked ubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RNF216
CAHH, TRIAD3, U7I1, UBCE7IP1, ZIN
ring finger protein 216
GO Process (4)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.
Ubiquitination is initiated by transfer of ubiquitin (Ub) from a ubiquitin-activating enzyme (E1) to a ubiquitin-conjugating enzyme (E2), producing a covalently linked intermediate (E2-Ub) 1 . Ubiquitin ligases (E3s) of the 'really interesting new gene' (RING) class recruit E2-Ub via their RING domain and then mediate direct transfer of ubiquitin to substrates 2 . By contrast, 'homologous to E6-AP carboxy ... [more]
Nature Dec. 01, 2017; 556(7701);381-385 [Pubmed: 29643511]
Throughput
- High Throughput
Additional Notes
- bait proteins from cell lysate were identified by mass spec in two separate experiments as proteins covalently attached via an aborted transthiolation attempt to a suicide substrate composed of both the E2s UBE2D2 and UBE2L3 attached in vitro to a tagged modified ubiquitin
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RNF216 UBE2D2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID