BAIT

STUB1

0610033N24Rik, 2210017D18Rik, 2310040B03Rik, AW046544, Chip
STIP1 homology and U-Box containing protein 1
GO Process (15)
GO Function (16)
GO Component (9)
Mus musculus
PREY

HSPA9

74kDa, Csa, Grp75, Hsc74, Hsp74, Hsp74a, Hspa9a, Mortalin, Mot-2, Mot2, Mthsp70, Pbp74
heat shock protein 9
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

UBXN2A enhances CHIP-mediated proteasomal degradation of oncoprotein mortalin-2 in cancer cells.

Sane S, Hafner A, Srinivasan R, Masood D, Slunecka JL, Noldner CJ, Hanson AD, Kruisselbrink T, Wang X, Wang Y, Yin J, Rezvani K

Overexpression of oncoproteins is a major cause of treatment failure using current chemotherapeutic drugs. Drug-induced degradation of oncoproteins is feasible and can improve clinical outcomes in diverse types of cancers. Mortalin-2 (mot-2) is a dominant oncoprotein in several tumors, including colorectal cancer (CRC). In addition to inactivating the p53 tumor suppressor protein, mot-2 enhances tumor cell invasion and migration. Thus, ... [more]

Mol Oncol Oct. 01, 2018; 12(10);1753-1777 [Pubmed: 30107089]

Throughput

  • Low Throughput

Curated By

  • BioGRID