Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Poly r(C) Binding Protein 1 Regulates Posttranscriptional Expression of the Ubiquitin Ligase TRIM56 in Ovarian Cancer.

Zhao L, Wang ZG, Zhang P, Yu XF, Su XJ

Our earlier work has shown that the E3 ligase TRIM56 messenger RNA (mRNA) level and vimentin protein expression followed an inverse correlation in ovarian carcinoma patients; however, the regulatory mechanisms underlying TRIM56 expression is unclear. Steady state expression of TRIM56 mRNA expression in the normal ovarian cell line Moody and ovarian cancer cell lines SKOV-3, A2780, and Caov-3 were not ... [more]

IUBMB Life Oct. 03, 2018; (); [Pubmed: 30281912]

Throughput

  • Low Throughput

Additional Notes

  • the TRIM56 3'UTR RNA was immunoprecipitated and PCBP1 was identified by mass spectrometry as an interacting protein

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PCBP1 TRIM56
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-

Curated By

  • BioGRID