Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor for its antiproliferative effects.

Zhang F, Baeumer N, Rode M, Ji P, Zhang T, Berdel WE, Mueller-Tidow C

The proteins of the Inhibitor of Growth (ING) family are involved in multiple cellular functions such as cell cycle regulation, apoptosis, and chromatin remodeling. For ING5, its actual role in growth suppression and the necessary partners are not known. In a yeast-two-hybrid approach with human bone marrow derived cDNA, we identified ING5 as well as several other proteins as interaction ... [more]

PLoS ONE Jul. 14, 2011; 6(7);e21505 [Pubmed: 21750715]

Throughput

  • Low Throughput

Additional Notes

  • data not shown

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
INCA1 RAB5C
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID