OPTN
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- Golgi organization [IMP]
- Golgi ribbon formation [IDA]
- Golgi to plasma membrane protein transport [IMP]
- cell death [TAS]
- defense response to Gram-negative bacterium [IMP]
- macroautophagy [IDA]
- mitotic cell cycle [TAS]
- negative regulation of receptor recycling [IMP]
- protein targeting to Golgi [IMP]
- regulation of I-kappaB kinase/NF-kappaB signaling [IBA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HTT
Gene Ontology Biological Process
- Golgi organization [IMP]
- establishment of mitotic spindle orientation [IMP]
- negative regulation of extrinsic apoptotic signaling pathway [IMP]
- organ development [IBA]
- positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA]
- regulation of protein phosphatase type 2A activity [IMP]
- retrograde vesicle-mediated transport, Golgi to ER [IMP]
- vesicle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Structural insights into the ubiquitin recognition by OPTN (optineurin) and its regulation by TBK1-mediated phosphorylation.
OPTN (optineurin), a ubiquitin-binding scaffold protein, functions as an important macroautophagy/autophagy receptor in selective autophagy processes. Mutations in OPTN have been linked with human neurodegenerative diseases including ALS and glaucoma. However, the mechanistic basis underlying the recognition of ubiquitin by OPTN and its regulation by TBK1-mediated phosphorylation are still elusive. Here, we demonstrate that the UBAN domain of OPTN preferentially ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
OPTN HTT | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
HTT OPTN | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3443689 | |
OPTN HTT | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - | |
OPTN HTT | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
HTT OPTN | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
OPTN HTT | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
HTT OPTN | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 823783 |
Curated By
- BioGRID