Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency.

Yu CY, Li TC, Wu YY, Yeh CH, Chiang W, Chuang CY, Kuo HC

Accumulating evidence indicates that circular RNAs (circRNAs) are abundant in the human transcriptome. However, their involvement in biological processes, including pluripotency, remains mostly undescribed. We identified a subset of circRNAs that are enriched in undifferentiated human embryonic stem cells (hESCs) and demonstrated that two, circBIRC6 and circCORO1C, are functionally associated with the pluripotent state. Mechanistically, we found that circBIRC6 is ... [more]

Nat Commun Dec. 27, 2016; 8(1);1149 [Pubmed: 29074849]

Throughput

  • Low Throughput

Additional Notes

  • circular BIRC6 RNA is co-immunoprecipitated when ESRP1 protein is pulled down

Curated By

  • BioGRID