Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes.

Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper JW, Howley PM

The E6AP ubiquitin-ligase catalyzes the high risk Human Papillomaviruses E6-mediated ubiquitylation of p53 contributing to the neoplastic progression of cells infected by these viruses. Defects in the activity and the dosage of E6AP are linked to Angelman Syndrome and to Autism Spectrum Disorders respectively, highlighting the need for precise control of this enzyme. With the exception of HERC2, which modulates ... [more]

Unknown May. 29, 2012; 0(0); [Pubmed: 22645313]

Throughput

  • High Throughput

Additional Notes

  • High-confidence candidate-interacting proteins (HCIPs) for wild type UBE3A/E6AP had NWD scores equal to or higher than 1 in both biological replicates.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UCHL5 UBE3A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9991BioGRID
1185563
UCHL5 UBE3A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9659BioGRID
2248652
UCHL5 UBE3A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9092BioGRID
3040669
UBE3A UCHL5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2515261
UCHL5 UBE3A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
UBE3A UCHL5
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-BioGRID
1057527

Curated By

  • BioGRID