NSD1
Gene Ontology Biological Process
Gene Ontology Molecular Function- androgen receptor binding [IDA]
- chromatin binding [ISS]
- estrogen receptor binding [ISS]
- histone methyltransferase activity (H3-K36 specific) [IDA, ISS]
- histone methyltransferase activity (H4-K20 specific) [ISS]
- retinoic acid receptor binding [ISS]
- retinoid X receptor binding [ISS]
- thyroid hormone receptor binding [ISS]
- transcription cofactor activity [IDA]
- transcription corepressor activity [ISS]
- zinc ion binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [ISS]
- estrogen receptor binding [ISS]
- histone methyltransferase activity (H3-K36 specific) [IDA, ISS]
- histone methyltransferase activity (H4-K20 specific) [ISS]
- retinoic acid receptor binding [ISS]
- retinoid X receptor binding [ISS]
- thyroid hormone receptor binding [ISS]
- transcription cofactor activity [IDA]
- transcription corepressor activity [ISS]
- zinc ion binding [IDA]
ZNF496
Gene Ontology Biological Process
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Nizp1, a novel multitype zinc finger protein that interacts with the NSD1 histone lysine methyltransferase through a unique C2HR motif.
Haploinsufficiency of the NSD1 gene is a hallmark of Sotos syndrome, and rearrangements of this gene by translocation can cause acute myeloid leukemia. The NSD1 gene product is a SET-domain histone lysine methyltransferase that has previously been shown to interact with nuclear receptors. We describe here a novel NSD1-interacting protein, Nizp1, that contains a SCAN box, a KRAB-A domain, and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NSD1 ZNF496 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NSD1 ZNF496 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| ZNF496 NSD1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID