BAIT

ZUFSP

C6orf113, dJ412I7.3, RP3-412I7.3
zinc finger with UFM1-specific peptidase domain
UPS Project
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

GAPDH

G3PD, GAPD, HEL-S-162eP, CDABP0047
glyceraldehyde-3-phosphate dehydrogenase
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes.

Hewings DS, Heideker J, Ma TP, AhYoung AP, El Oualid F, Amore A, Costakes GT, Kirchhofer D, Brasher B, Pillow T, Popovych N, Maurer T, Schwerdtfeger C, Forrest WF, Yu K, Flygare J, Bogyo M, Wertz IE

Activity-based probes (ABPs) are widely used to monitor the activity of enzyme families in biological systems. Inferring enzyme activity from probe reactivity requires that the probe reacts with the enzyme at its active site; however, probe-labeling sites are rarely verified. Here we present an enhanced chemoproteomic approach to evaluate the activity and probe reactivity of deubiquitinase enzymes, using bioorthogonally tagged ... [more]

Nat Commun Mar. 21, 2018; 9(1);1162 [Pubmed: 29563501]

Throughput

  • High Throughput

Curated By

  • BioGRID