Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rab5 is a signalling GTPase involved in actin remodelling by receptor tyrosine kinases.

Lanzetti L, Palamidessi A, Areces L, Scita G, Di Fiore PP

Rab5 is a small GTPase involved in the control of intracellular trafficking, both at the level of receptor endocytosis and endosomal dynamics. The finding that Rab5 can be activated by receptor tyrosine kinases (RTK) raised the question of whether it also participates in effector pathways emanating from these receptors. Here we show that Rab5 is indispensable for a form of ... [more]

Nature May. 20, 2004; 429(6989);309-14 [Pubmed: 15152255]

Throughput

  • Low Throughput

Curated By

  • BioGRID