An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Synthesis of Isomeric Phosphoubiquitin Chains Reveals that Phosphorylation Controls Deubiquitinase Activity and Specificity.

Huguenin-Dezot N, De Cesare V, Peltier J, Knebel A, Kristaryianto YA, Rogerson DT, Kulathu Y, Trost M, Chin JW

Ubiquitin is post-translationally modified by phosphorylation at several sites, but the consequences of these modifications are largely unknown. Here, we synthesize multi-milligram quantities of ubiquitin phosphorylated at serine 20, serine 57, and serine 65 via genetic code expansion. We use these phosphoubiquitins for the enzymatic assembly of 20 isomeric phosphoubiquitin dimers, with different sites of isopeptide linkage and/or phosphorylation. We ... [more]

Cell Rep Dec. 26, 2015; 16(4);1180-1193 [Pubmed: 27425610]

Throughput

Low Throughput

Additional Notes

ubiquitin transthiolation

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBE2H UBA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Chrustowicz J (2024)	High	-	BioGRID	-
UBA1 UBE2H	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Koszela J (2018)	Low	-	BioGRID	2534161
UBA1 UBE2H	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Jin J (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UBA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin activating enzyme activity [IBA, IDA]ubiquitin-protein transferase activity [IBA]

Gene Ontology Cellular Component

UBE2H

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]small conjugating protein transferase activity [IBA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [IDA]