EP300
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- N-terminal peptidyl-lysine acetylation [IDA]
- Notch signaling pathway [TAS]
- apoptotic process [IMP]
- cellular response to hypoxia [TAS]
- chromatin organization [TAS]
- circadian rhythm [ISS]
- histone H2B acetylation [IDA]
- histone H4 acetylation [IMP]
- innate immune response [TAS]
- internal peptidyl-lysine acetylation [IDA]
- internal protein amino acid acetylation [IDA]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IDA]
- mitotic cell cycle [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- nervous system development [TAS]
- positive regulation by host of viral transcription [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response [ISS]
- positive regulation of type I interferon production [TAS]
- protein stabilization [ISS]
- regulation of androgen receptor signaling pathway [IDA]
- regulation of cell cycle [TAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- regulation of transcription, DNA-templated [IDA]
- regulation of tubulin deacetylation [IDA]
- response to estrogen [IDA]
- response to hypoxia [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- acetyltransferase activity [IDA, IMP]
- activating transcription factor binding [IPI]
- androgen receptor binding [IPI]
- beta-catenin binding [IPI]
- chromatin binding [IMP]
- core promoter binding [IDA]
- histone acetyltransferase activity [IDA]
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [IDA]
- nuclear hormone receptor binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transferase activity, transferring acyl groups [IDA]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- acetyltransferase activity [IDA, IMP]
- activating transcription factor binding [IPI]
- androgen receptor binding [IPI]
- beta-catenin binding [IPI]
- chromatin binding [IMP]
- core promoter binding [IDA]
- histone acetyltransferase activity [IDA]
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [IDA]
- nuclear hormone receptor binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transferase activity, transferring acyl groups [IDA]
CITED2
Gene Ontology Biological Process
- adrenal cortex formation [ISS]
- cell proliferation [IDA]
- determination of left/right symmetry [ISS]
- embryonic heart tube left/right pattern formation [ISS]
- heart development [IMP, ISS]
- left/right axis specification [ISS]
- liver development [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of cell migration [IMP]
- negative regulation of gene expression [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry [ISS]
- outflow tract morphogenesis [ISS]
- positive regulation of cell cycle [ISS]
- positive regulation of cell-cell adhesion [ISS]
- positive regulation of gene expression [IDA]
- positive regulation of male gonad development [ISS]
- positive regulation of peroxisome proliferator activated receptor signaling pathway [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISS]
- positive regulation of transcription, DNA-templated [IDA, ISS]
- positive regulation of transforming growth factor beta receptor signaling pathway [ISS]
- regulation of nucleic acid-templated transcription [IDA]
- regulation of organ formation [ISS]
- response to estrogen [IDA]
- response to fluid shear stress [IMP]
- response to hypoxia [IDA, IMP]
- sex determination [ISS]
- spleen development [ISS]
- ventricular septum morphogenesis [ISS]
Gene Ontology Molecular Function- LBD domain binding [IPI]
- RNA polymerase II transcription corepressor activity [IDA]
- chromatin binding [ISS]
- histone acetyltransferase binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS]
- transcription coactivator activity [IDA]
- transcription corepressor activity [IDA, IMP]
- LBD domain binding [IPI]
- RNA polymerase II transcription corepressor activity [IDA]
- chromatin binding [ISS]
- histone acetyltransferase binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS]
- transcription coactivator activity [IDA]
- transcription corepressor activity [IDA, IMP]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2.
The transcriptional co-activators and histone acetyltransferases p300/CREB-binding protein (CBP) interact with CITED2, a transcription factor AP-2 (TFAP2) co-activator. p300/CBP, CITED2, and TFAP2A are essential for normal neural tube and cardiac development. Here we show that p300 and CBP co-activate TFAP2A in the presence of CITED2. TFAP2A transcriptional activity was modestly impaired in p300(+/-) and CBP(+/-) mouse embryonic fibroblasts; this was ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EP300 CITED2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1450449 | |
| EP300 CITED2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CITED2 EP300 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 1513562 | |
| EP300 CITED2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| EP300 CITED2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| CITED2 EP300 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| CITED2 EP300 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| EP300 CITED2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| EP300 CITED2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| CITED2 EP300 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID