Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FGF11 induced by hypoxia interacts with HIF-1α and enhances its stability.

Lee KW, Yim HS, Shin J, Lee C, Lee JH, Jeong JY

Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an interacting protein of FGF11 using immunoprecipitation and mass spectrometry. FGF11 knockdown decreased ... [more]

FEBS Lett. Dec. 01, 2016; 591(2);348-357 [Pubmed: 28027390]

Throughput

  • Low Throughput

Curated By

  • BioGRID