BAIT

UTF1

AI505934
undifferentiated embryonic cell transcription factor 1
GO Process (1)
GO Function (3)
GO Component (1)

Gene Ontology Cellular Component

Mus musculus
PREY

MYC

AU016757, Myc2, Niard, Nird, bHLHe39
myelocytomatosis oncogene
GO Process (67)
GO Function (13)
GO Component (11)

Gene Ontology Biological Process

Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Myc binds the pluripotency factor Utf1 through the basic-helix-loop-helix leucine zipper domain.

Laskowski AI, Knoepfler PS

In order to elucidate the function of Myc in the maintenance of pluripotency and self-renewal in mouse embryonic stem cells (mESCs), we screened for novel ESC-specific interactors of Myc by mass spectrometry. Undifferentiated embryonic cell transcription factor 1 (Utf1) was identified in the screen as a putative Myc binding protein in mESCs. We found that Myc and Utf1 directly interact. ... [more]

Biochem. Biophys. Res. Commun. Jun. 14, 2013; 435(4);551-6 [Pubmed: 23665319]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MYC UTF1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID