BAIT

RNU11

RNU11-1, U11
RNA, U11 small nuclear
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

PRKRIP1

C114, KRBOX3
PRKR interacting protein 1 (IL11 inducible)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.

Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Luehrmann R

U11 and U12 snRNPs bind U12-type pre-mRNAs as a preformed di-snRNP complex, simultaneously recognizing the 5' splice site and branchpoint sequence. Thus, within the U12-type prespliceosome, U11/U12 components form a molecular bridge connecting both ends of the intron. We have affinity purified human 18S U11/U12 and 12S U11 snRNPs, and identified their protein components by using mass spectrometry. U11/U12 snRNPs ... [more]

RNA Jun. 01, 2004; 10(6);929-41 [Pubmed: 15146077]

Throughput

  • Low Throughput

Curated By

  • BioGRID