An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phactrs 1-4: A family of protein phosphatase 1 and actin regulatory proteins.

Allen PB, Greenfield AT, Svenningsson P, Haspeslagh DC, Greengard P

Protein phosphatase 1 (PP1) is a multifunctional enzyme with diverse roles in the nervous system, including regulation of synaptic activity and dendritic morphology. PP1 activity is controlled via association with a family of regulatory subunits that govern subcellular localization and substrate specificity. A previously undescribed class of PP1-binding proteins was detected by interaction cloning. Family members were also found to ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 04, 2004; 101(18);7187-92 [Pubmed: 15107502]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PHACTR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [ISS]

Gene Ontology Cellular Component

ACTA1