BAIT

PRE1

proteasome core particle subunit beta 4, L000001483, YER012W
Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

VMA16

PPA1, H(+)-transporting V0 sector ATPase subunit c'', L000001468, YHR026W
Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

The proteasomal substrate Stm1 participates in apoptosis-like cell death in yeast.

Ligr M, Velten I, Froehlich E, Madeo F, Ledig M, Froehlich KU, Wolf DH, Hilt W

We have identified the yeast gene STM1 in an overexpression screen for new proteasomal substrates. Stm1 is unstable in wild-type cells and stabilized in cells with defective proteasomal activity and thus a bona fide substrate of the proteasome. It is localized in the perinuclear region and is required for growth in the presence of mutagens. Overexpression in cells with impaired ... [more]

Mol. Biol. Cell Aug. 01, 2001; 12(8);2422-32 [Pubmed: 11514626]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • in pre4 background

Curated By

  • BioGRID