BAIT

PRE4

proteasome core particle subunit beta 7, L000001486, YFR050C
Beta 7 subunit of the 20S proteasome
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

STM1

MPT4, L000002574, YLR150W
Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

The proteasomal substrate Stm1 participates in apoptosis-like cell death in yeast.

Ligr M, Velten I, Froehlich E, Madeo F, Ledig M, Froehlich KU, Wolf DH, Hilt W

We have identified the yeast gene STM1 in an overexpression screen for new proteasomal substrates. Stm1 is unstable in wild-type cells and stabilized in cells with defective proteasomal activity and thus a bona fide substrate of the proteasome. It is localized in the perinuclear region and is required for growth in the presence of mutagens. Overexpression in cells with impaired ... [more]

Mol. Biol. Cell Aug. 01, 2001; 12(8);2422-32 [Pubmed: 11514626]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • in pre1 background

Curated By

  • BioGRID