ITCH
Gene Ontology Biological Process
- negative regulation of JNK cascade [IMP]
- negative regulation of NF-kappaB transcription factor activity [IMP]
- negative regulation of alpha-beta T cell proliferation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of defense response to virus [IMP, ISO]
- positive regulation of T cell anergy [IMP]
- positive regulation of protein catabolic process [IDA, IMP]
- protein K29-linked ubiquitination [ISO]
- protein K48-linked ubiquitination [IMP, ISO]
- protein K63-linked ubiquitination [ISO]
- protein polyubiquitination [IDA]
- protein ubiquitination [IBA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of protein deubiquitination [IMP]
- ubiquitin-dependent protein catabolic process [IDA, ISA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARRB2
Gene Ontology Biological Process
- G-protein coupled receptor internalization [ISO]
- G-protein coupled receptor signaling pathway [ISO]
- adult walking behavior [IMP]
- cell chemotaxis [ISO]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [ISO]
- detection of temperature stimulus involved in sensory perception of pain [ISO]
- endocytosis [ISO]
- follicle-stimulating hormone signaling pathway [ISO]
- negative regulation of GTPase activity [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of interleukin-1 beta production [IMP]
- negative regulation of interleukin-12 production [IMP]
- negative regulation of interleukin-6 production [IMP]
- negative regulation of natural killer cell mediated cytotoxicity [ISO]
- negative regulation of protein ubiquitination [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO]
- negative regulation of smooth muscle cell apoptotic process [ISO]
- negative regulation of toll-like receptor signaling pathway [IMP]
- negative regulation of tumor necrosis factor production [IMP]
- positive regulation of DNA biosynthetic process [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of calcium ion transport [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of protein kinase B signaling [IMP, ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of protein ubiquitination [ISO]
- positive regulation of receptor internalization [ISO]
- positive regulation of synaptic transmission, dopaminergic [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein ubiquitination [ISO]
- receptor internalization [ISO]
- regulation of G-protein coupled receptor protein signaling pathway [IMP, ISO]
- regulation of androgen receptor signaling pathway [ISO]
- regulation of protein phosphorylation [IDA, IGI]
- transcription from RNA polymerase II promoter [ISO]
- transforming growth factor beta receptor signaling pathway [ISO]
Gene Ontology Molecular Function- 14-3-3 protein binding [ISO]
- D1 dopamine receptor binding [ISO]
- G-protein coupled receptor binding [ISO]
- alpha-1A adrenergic receptor binding [ISO]
- alpha-1B adrenergic receptor binding [ISO]
- angiotensin receptor binding [ISO]
- enzyme binding [ISO]
- follicle-stimulating hormone receptor binding [ISO]
- mitogen-activated protein kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein complex scaffold [ISO]
- protein domain specific binding [ISO]
- protein kinase B binding [IPI]
- receptor binding [ISO]
- type 1 angiotensin receptor binding [ISO]
- type 2A serotonin receptor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- 14-3-3 protein binding [ISO]
- D1 dopamine receptor binding [ISO]
- G-protein coupled receptor binding [ISO]
- alpha-1A adrenergic receptor binding [ISO]
- alpha-1B adrenergic receptor binding [ISO]
- angiotensin receptor binding [ISO]
- enzyme binding [ISO]
- follicle-stimulating hormone receptor binding [ISO]
- mitogen-activated protein kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein complex scaffold [ISO]
- protein domain specific binding [ISO]
- protein kinase B binding [IPI]
- receptor binding [ISO]
- type 1 angiotensin receptor binding [ISO]
- type 2A serotonin receptor binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis.
Suppressor of Fused (SuFu), a tumour suppressor mutated in medulloblastoma, is a central player of Hh signalling, a pathway crucial for development and deregulated in cancer. Although the control of Gli transcription factors by SuFu is critical in Hh signalling, our understanding of the mechanism regulating this key event remains limited. Here, we show that the Itch/β-arrestin2 complex binds SuFu ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ITCH ARRB2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.828 | BioGRID | 2666945 |
Curated By
- BioGRID