Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic identification of brain proteins that interact with dynein light chain LC8.

Navarro-Lerida I, Martinez Moreno M, Roncal F, Gavilanes F, Albar JP, Rodriguez-Crespo I

Cytoplasmic dynein is a large minus end-directed microtubule motor that translocates cargos towards the minus end of microtubules. Light chain 8 of the dynein machinery (LC8) has been reported to interact with a large variety of proteins that possess K/RSTQT or GIQVD motifs in their sequence, hence permitting their transport in a retrograde manner. Yeast two-hybrid analysis has revealed that ... [more]

Proteomics Feb. 01, 2004; 4(2);339-46 [Pubmed: 14760703]

Throughput

  • Low Throughput

Curated By

  • BioGRID