RPTOR
Gene Ontology Biological Process
- TOR signaling [ISO]
- cell growth [ISO]
- cellular response to amino acid stimulus [ISO]
- cellular response to nutrient levels [ISO]
- positive regulation of TOR signaling [ISO]
- positive regulation of endothelial cell proliferation [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of transcription from RNA polymerase III promoter [ISO]
- regulation of cell size [ISO]
- regulation of phosphorylation [ISO]
Gene Ontology Molecular Function- 14-3-3 protein binding [ISO]
- RNA polymerase III type 1 promoter DNA binding [ISO]
- RNA polymerase III type 2 promoter DNA binding [ISO]
- RNA polymerase III type 3 promoter DNA binding [ISO]
- TFIIIC-class transcription factor binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- 14-3-3 protein binding [ISO]
- RNA polymerase III type 1 promoter DNA binding [ISO]
- RNA polymerase III type 2 promoter DNA binding [ISO]
- RNA polymerase III type 3 promoter DNA binding [ISO]
- TFIIIC-class transcription factor binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
Gene Ontology Cellular Component
AKT1S1
Gene Ontology Biological Process
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Proteomic analysis reveals GIT1 as a novel mTOR complex component critical for mediating astrocyte survival.
As a critical regulator of cell growth, the mechanistic target of rapamycin (mTOR) protein operates as part of two molecularly and functionally distinct complexes. Herein, we demonstrate that mTOR complex molecular composition varies in different somatic tissues. In astrocytes and neural stem cells, we identified G-protein-coupled receptor kinase-interacting protein 1 (GIT1) as a novel mTOR-binding protein, creating a unique mTOR ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPTOR AKT1S1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID