Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The interaction of mammalian Class C Vps with nSec-1/Munc18-a and syntaxin 1A regulates pre-synaptic release.

Kim BY, Sahara Y, Yamamoto A, Kominami E, Kohsaka S, Akazawa C

Membrane docking and fusion in neurons is a highly regulated process requiring the participation of a large number of SNAREs (soluble N-ethylmaleimide sensitive factor attachment protein receptors) and SNARE-interacting proteins. We found that mammalian Class C Vps protein complex associated specifically with nSec-1/Munc18-a, and syntaxin 1A both in vivo and in vitro. In contrast, VAMP2 and SNAP-25, other neuronal core ... [more]

Biochem. Biophys. Res. Commun. Nov. 24, 2006; 350(3);691-7 [Pubmed: 17027648]

Throughput

  • Low Throughput

Curated By

  • BioGRID