An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Receptor-type protein-tyrosine phosphatase Î¶ is a functional receptor for interleukin-34.

Nandi S, Cioce M, Yeung YG, Nieves E, Tesfa L, Lin H, Hsu AW, Halenbeck R, Cheng HY, Gokhan S, Mehler MF, Stanley ER

Interleukin-34 (IL-34) is highly expressed in brain. IL-34 signaling via its cognate receptor, colony-stimulating factor-1 receptor (CSF-1R), is required for the development of microglia. However, the differential expression of IL-34 and the CSF-1R in brain suggests that IL-34 may signal via an alternate receptor. By IL-34 affinity chromatography of solubilized mouse brain membrane followed by mass spectrometric analysis, we identified ... [more]

J. Biol. Chem. Jul. 26, 2013; 288(30);21972-86 [Pubmed: 23744080]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PTPRZ1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein tyrosine phosphatase activity [ISS]transmembrane receptor protein tyrosine phosphatase activity [TAS]

Gene Ontology Cellular Component

IL34