An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cloning and characterization of mCRIP2, a mouse LIM-only protein that interacts with PDZ domain IV of PTP-BL.

van Ham M, Croes H, Schepens J, Fransen J, Wieringa B, Hendriks W

BACKGROUND: In the mouse submembranous protein tyrosine phosphatase PTP-BL five PDZ domains are present in between the N-terminal FERM domain, which directs the protein to the cell cortex, and the C-terminal catalytic phosphatase domain. To understand more on the physical role of PTP-BL in this microenvironment, we started to search for PTP-BL PDZ domain-interacting proteins. RESULTS: Yeast two-hybrid screening for ... [more]

Genes Cells Jul. 01, 2003; 8(7);631-44 [Pubmed: 12839623]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PTPN13 CRIP2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	van Ham M (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PTPN13

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphatidylinositol 3-kinase regulatory subunit binding [ISO]protein binding [IPI]protein tyrosine phosphatase activity [ISO]

Gene Ontology Cellular Component

CRIP2