PREY

AXL

AI323647, Ark, Tyro7, Ufo
AXL receptor tyrosine kinase
GO Process (30)
GO Function (4)
GO Component (5)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A mammalian adaptor protein with conserved Src homology 2 and phosphotyrosine-binding domains is related to Shc and is specifically expressed in the brain.

O'Bryan JP, Songyang Z, Cantley L, Der CJ, Pawson T

The Shc adaptor protein, hereafter referred to as ShcA, possesses two distinct phosphotyrosine-recognition modules, a C-terminal Src homology 2 (SH2) domain and an N-terminal phosphotyrosine-binding (PTB) domain, and is itself phosphorylated on tyrosine in response to many extracellular signals. Phosphorylation of human ShcA at Tyr-317 within its central (CH1) region induces binding to the Grb2 SH2 domain and is thereby ... [more]

Proc. Natl. Acad. Sci. U.S.A. Apr. 02, 1996; 93(7);2729-34 [Pubmed: 8610109]

Throughput

  • Low Throughput

Curated By

  • BioGRID