An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Neural recognition molecules CHL1 and NB-3 regulate apical dendrite orientation in the neocortex via PTP alpha.

Ye H, Tan YL, Ponniah S, Takeda Y, Wang SQ, Schachner M, Watanabe K, Pallen CJ, Xiao ZC

Apical dendrites of pyramidal neurons in the neocortex have a stereotypic orientation that is important for neuronal function. Neural recognition molecule Close Homolog of L1 (CHL1) has been shown to regulate oriented growth of apical dendrites in the mouse caudal cortex. Here we show that CHL1 directly associates with NB-3, a member of the F3/contactin family of neural recognition molecules, ... [more]

EMBO J. Jan. 09, 2008; 27(1);188-200 [Pubmed: 18046458]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PTPRA

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein tyrosine phosphatase activity [TAS]transmembrane receptor protein tyrosine phosphatase activity [TAS]

Gene Ontology Cellular Component

CHL1