BAIT

CA2

CA-II, CAC, CAII, Car2, HEL-76
carbonic anhydrase II
Homo sapiens
PREY

SLC4A8

NBC3, NDCBE
solute carrier family 4, sodium bicarbonate cotransporter, member 8
GO Process (3)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Regulation of the human NBC3 Na+/HCO3- cotransporter by carbonic anhydrase II and PKA.

Loiselle FB, Morgan PE, Alvarez BV, Casey JR

Human NBC3 is an electroneutral Na(+)/HCO(3)(-) cotransporter expressed in heart, skeletal muscle, and kidney in which it plays an important role in HCO(3)(-) metabolism. Cytosolic enzyme carbonic anhydrase II (CAII) catalyzes the reaction CO(2) + H(2)O left arrow over right arrow HCO(3)(-) + H(+) in many tissues. We investigated whether NBC3, like some Cl(-)/HCO(3)(-) exchange proteins, could bind CAII and ... [more]

Am. J. Physiol., Cell Physiol. Jun. 01, 2004; 286(6);C1423-33 [Pubmed: 14736710]

Throughput

  • Low Throughput

Curated By

  • BioGRID