BAIT

HSPH1

HSP105, HSP105A, HSP105B, NY-CO-25, RP11-173P16.1
heat shock 105kDa/110kDa protein 1
Alzheimer's Disease Project
Homo sapiens
PREY

UBA1

A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, CTD-2522E6.1
ubiquitin-like modifier activating enzyme 1
UPS Project
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Screening of Hsp105alpha-binding proteins using yeast and bacterial two-hybrid systems.

Saito Y, Doi K, Yamagishi N, Ishihara K, Hatayama T

Hsp105alpha is a 105-kDa stress protein, which is expressed constitutively at especially high levels in the brain compared with other tissues in mammals, and is also induced by a variety of stressors. Recently, we have shown that Hsp105alpha binds to alpha-tubulin and prevents the heat-induced disaggregation of microtubules. To further elucidate the function of Hsp105alpha, we searched for Hsp105alpha-binding proteins ... [more]

Biochem. Biophys. Res. Commun. Feb. 06, 2004; 314(2);396-402 [Pubmed: 14733918]

Throughput

  • Low Throughput

Curated By

  • BioGRID