Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins.

Khanna R, Krishnamoorthy V, Parnaik VK

Lamins are key nuclear proteins which are important for maintaining nuclear structure and function. Mutations in lamins cause a spectrum of genetic diseases termed as laminopathies. RING finger containing E3 ubiquitin ligase, RNF123, is transcriptionally upregulated in cells expressing rod domain lamin A mutations. However, the functional relevance of RNF123 in laminopathic cells is not clear. Using a mass spectrometry-based ... [more]

FEBS J. Jun. 01, 2018; 285(12);2243-2262 [Pubmed: 29676528]

Throughput

  • High Throughput

Curated By

  • BioGRID