BAIT

PPT1

CLN1, INCL, PPT, RP11-115D7.2
palmitoyl-protein thioesterase 1
Homo sapiens
PREY

EEF1A2

EEF1AL, EF-1-alpha-2, EF1A, HS1, STN, STNL
eukaryotic translation elongation factor 1 alpha 2
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative analysis of PPT1 interactome in human neuroblastoma cells.

Scifo E, Szwajda A, Soliymani R, Pezzini F, Bianchi M, Dapkunas A, Debski J, Uusi-Rauva K, Dadlez M, Gingras AC, Tyynelae J, Simonati A, Jalanko A, Baumann MH, Lalowski M

Mutations in the CLN1 gene that encodes Palmitoyl protein thioesterase 1 (PPT1) or CLN1, cause Infantile NCL (INCL, MIM#256730). PPT1 removes long fatty acid chains such as palmitate from modified cysteine residues of proteins. The data shown here result from isolated protein complexes from PPT1-expressing SH-SY5Y stable cells that were subjected to single step affinity purification coupled to mass spectrometry ... [more]

Data Brief Sep. 01, 2015; 4();207-16 [Pubmed: 26217791]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PPT1 EEF1A2
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3745563

Curated By

  • BioGRID