Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Insights into the ubiquitin-proteasome system of human embryonic stem cells.

Saez I, Koyuncu S, Gutierrez-Garcia R, Dieterich C, Vilchez D

Human embryonic stem cells (hESCs) exhibit high levels of proteasome activity, an intrinsic characteristic required for their self-renewal, pluripotency and differentiation. However, the mechanisms by which enhanced proteasome activity maintains hESC identity are only partially understood. Besides its essential role for the ability of hESCs to suppress misfolded protein aggregation, we hypothesize that enhanced proteasome activity could also be important ... [more]

Sci Rep Mar. 06, 2018; 8(1);4092 [Pubmed: 29511261]

Throughput

  • High Throughput

Additional Notes

  • High confidence UBR7 interactors

Curated By

  • BioGRID