HYAL2
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cartilage development [IEP]
- cellular response to UV-B [IDA]
- cellular response to fibroblast growth factor stimulus [IDA]
- cellular response to interleukin-1 [IDA]
- cellular response to transforming growth factor beta stimulus [IDA]
- cellular response to tumor necrosis factor [IEP]
- fusion of virus membrane with host plasma membrane [IDA]
- glycosaminoglycan catabolic process [IDA]
- glycosaminoglycan metabolic process [TAS]
- hyaluronan catabolic process [IDA, TAS]
- hyaluronan metabolic process [TAS]
- kidney development [ISS]
- monocyte activation [IDA]
- negative regulation of MAP kinase activity [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of fibroblast migration [IDA]
- negative regulation of protein kinase B signaling [IDA]
- negative regulation of protein tyrosine kinase activity [IDA]
- positive regulation of extrinsic apoptotic signaling pathway [ISS]
- positive regulation of inflammatory response [IDA]
- positive regulation of interleukin-6 secretion [IDA]
- positive regulation of interleukin-8 secretion [IDA]
- positive regulation of protein import into nucleus [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of urine volume [ISS]
- renal water absorption [ISS]
- response to antibiotic [IEP]
- response to reactive oxygen species [IDA]
- response to virus [IDA]
- small molecule metabolic process [TAS]
- transformation of host cell by virus [IDA, ISS]
- viral entry into host cell [ISS]
Gene Ontology Molecular Function- enzyme binding [IPI]
- hyaluronic acid binding [IDA]
- hyaluronoglucuronidase activity [IDA]
- hyalurononglucosaminidase activity [IDA, TAS]
- protein binding [IPI]
- receptor signaling protein tyrosine kinase inhibitor activity [IDA]
- receptor tyrosine kinase binding [IPI]
- transforming growth factor beta binding [ISS]
- virus receptor activity [IDA]
- enzyme binding [IPI]
- hyaluronic acid binding [IDA]
- hyaluronoglucuronidase activity [IDA]
- hyalurononglucosaminidase activity [IDA, TAS]
- protein binding [IPI]
- receptor signaling protein tyrosine kinase inhibitor activity [IDA]
- receptor tyrosine kinase binding [IPI]
- transforming growth factor beta binding [ISS]
- virus receptor activity [IDA]
Gene Ontology Cellular Component
- Golgi membrane [ISS]
- RNA polymerase II transcription factor complex [ISS]
- anchored component of external side of plasma membrane [IDA]
- anchored component of plasma membrane [IDA]
- apical plasma membrane [IDA]
- cell surface [IDA, ISS]
- cytoplasm [IDA]
- cytoplasmic membrane-bounded vesicle [IDA]
- cytoplasmic vesicle [IDA]
- cytosol [IDA]
- endocytic vesicle [IDA]
- endoplasmic reticulum [NAS]
- lysosome [IDA]
- membrane raft [IDA]
- microvillus [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [TAS]
SMAD4
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- SMAD protein complex assembly [IDA]
- SMAD protein signal transduction [IDA]
- gene expression [TAS]
- intracellular signal transduction [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of protein catabolic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- palate development [ISS]
- positive regulation of BMP signaling pathway [IMP]
- positive regulation of SMAD protein import into nucleus [ISS]
- positive regulation of epithelial to mesenchymal transition [ISS]
- positive regulation of pathway-restricted SMAD protein phosphorylation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, TAS]
- positive regulation of transcription, DNA-templated [IDA]
- positive regulation of transforming growth factor beta receptor signaling pathway [IDA]
- regulation of transforming growth factor beta receptor signaling pathway [IMP]
- regulation of transforming growth factor beta2 production [IMP]
- response to hypoxia [IMP]
- response to transforming growth factor beta [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IDA, TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, common-partner cytoplasmic mediator activity [IDA]
- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, common-partner cytoplasmic mediator activity [IDA]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Hyaluronan activates Hyal-2/WWOX/Smad4 signaling and causes bubbling cell death when the signaling complex is overexpressed.
Malignant cancer cells frequently secrete significant amounts of transforming growth factor beta (TGF-β), hyaluronan (HA) and hyaluronidases to facilitate metastasizing to target organs. In a non-canonical signaling, TGF-β binds membrane hyaluronidase Hyal-2 for recruiting tumor suppressors WWOX and Smad4, and the resulting Hyal-2/WWOX/Smad4 complex is accumulated in the nucleus to enhance SMAD-promoter dependent transcriptional activity. Yeast two-hybrid analysis showed that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HYAL2 SMAD4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID