BAIT

ERG2

END11, C-8 sterol isomerase ERG2, L000000569, L000004560, YMR202W

C-8 sterol isomerase; catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis

GO Process (1)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

ergosterol biosynthetic process [IMP]

Gene Ontology Molecular Function

C-8 sterol isomerase activity [IMP]

Gene Ontology Cellular Component

endoplasmic reticulum [IC]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

ELO3

SUR4, APA1, SRE1, VBM1, fatty acid elongase ELO3, L000002245, YLR372W

Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

A mutation in sphingolipid synthesis suppresses defects in yeast ergosterol metabolism.

Valachovic M, Wilcox LI, Sturley SL, Bard M

A mutation in an otherwise nonessential ERG2 gene is synthetically lethal when combined with mutations in two transcription factors encoded by the UPC2 and ECM22 genes. Employing UV mutagenesis, we isolated a suppressor of the triple mutant erg2delta upc2delta ecm22delta. The morpholine-resistant phenotype of the suppressor was used to identify the suppressor as a mutation in the ELO3 gene. In ... [more]

Lipids Aug. 01, 2004; 39(8);747-52 [Pubmed: 15638242]

Throughput

Low Throughput

Ontology Terms

phenotype: viability (APO:0000111)

Additional Notes

erg2 upc2 ecm22 triple mutant

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ELO3 ERG2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	4	BioGRID	3603635
ERG2 ELO3	Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Jonikas MC (2009)	High	-	BioGRID	460842
ELO3 ERG2	Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Jonikas MC (2009)	High	-	BioGRID	459508
ERG2 ELO3	Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Shah Alam Bhuiyan M (2007)	Low	-	BioGRID	238011
ELO3 ERG2	Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.	Szappanos B (2011)	High	0.3092	BioGRID	536373
ERG2 ELO3	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Silve S (1996)	Low	-	BioGRID	260346

Curated By

BioGRID

Interaction Summary