BAIT

SOD1

ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer
superoxide dismutase 1, soluble
GO Process (50)
GO Function (9)
GO Component (17)

Gene Ontology Biological Process

Homo sapiens
PREY

HECW1

NEDL1
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
GO Process (1)
GO Function (1)
GO Component (0)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1.

Miyazaki K, Fujita T, Ozaki T, Kato C, Kurose Y, Sakamoto M, Kato S, Goto T, Itoyama Y, Aoki M, Nakagawara A

Approximately 20% of familial amyotrophic lateral sclerosis (FALS) arises from germ-line mutations in the superoxide dismutase-1 (SOD1) gene. However, the molecular mechanisms underlying the process have been elusive. Here, we show that a neuronal homologous to E6AP carboxyl terminus (HECT)-type ubiquitin-protein isopeptide ligase (NEDL1) physically binds translocon-associated protein-delta and also binds and ubiquitinates mutant (but not wild-type) SOD1 proportionately to ... [more]

J. Biol. Chem. Mar. 19, 2004; 279(12);11327-35 [Pubmed: 14684739]

Throughput

  • Low Throughput

Curated By

  • BioGRID