Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Activation of ferritinophagy is required for the RNA-binding protein ELAVL1/HuR to regulate ferroptosis in hepatic stellate cells.

Zhang Z, Yao Z, Wang L, Ding H, Shao J, Chen A, Zhang F, Zheng S

Ferroptosis is a recently recognized form of regulated cell death that is characterized by lipid peroxidation. However, the molecular mechanisms regulating ferroptosis are largely unknown. In this study, we report that the RNA-binding protein ELAVL1/HuR plays a crucial role in regulating ferroptosis in liver fibrosis. Upon exposure to ferroptosis-inducing compounds, ELAVL1 protein expression was remarkably increased through the inhibition of ... [more]

Autophagy Aug. 08, 2018; 14(12);2083-2103 [Pubmed: 30081711]

Throughput

  • Low Throughput

Additional Notes

  • when ELAVL1 protein is pulled down from cell lysates, BECN1 RNA is co-precipitated

Curated By

  • BioGRID