Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

LZTR1 is a regulator of RAS ubiquitination and signaling.

Bigenzahn JW, Collu GM, Kartnig F, Pieraks M, Vladimer GI, Heinz LX, Sedlyarov V, Schischlik F, Fauster A, Rebsamen M, Parapatics K, Blomen VA, Mueller AC, Winter GE, Kralovics R, Brummelkamp TR, Mlodzik M, Superti-Furga G

In genetic screens aimed at understanding drug resistance mechanisms in chronic myeloid leukemia cells, inactivation of the cullin 3 adapter protein-encoding leucine zipper-like transcription regulator 1 (LZTR1) gene led to enhanced mitogen-activated protein kinase (MAPK) pathway activity and reduced sensitivity to tyrosine kinase inhibitors. Knockdown of the Drosophila LZTR1 ortholog CG3711 resulted in a Ras-dependent gain-of-function phenotype. Endogenous human LZTR1 ... [more]

Science Dec. 07, 2017; 362(6419);1171-1177 [Pubmed: 30442766]

Throughput

  • High Throughput

Additional Notes

  • prey proteins were identified with an avgP score of 1 in high throughput BioID proteomic screens using HRAS, KRAS4A, KRAS4B and NRAS as baits

Curated By

  • BioGRID