PREY

F8

AHF, DXS1253E, F8B, F8C, FVIII, HEMA, RP11-115M6.7
coagulation factor VIII, procoagulant component
GO Process (4)
GO Function (1)
GO Component (3)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

LMAN1 is a molecular chaperone for the secretion of coagulation factor VIII.

Cunningham MA, Pipe SW, Zhang B, Hauri HP, Ginsburg D, Kaufman RJ

Combined deficiency of both coagulation factors (F)V and VIII is a rare autosomal recessive bleeding disorder caused by null expression of LMAN1 (previously termed ERGIC-53) in a majority of affected individuals. Previously, a requirement for a functional LMAN1 cycling pathway between the ER and Golgi was demonstrated for efficient secretion of FV and FVIII (Moussalli et al. J Biol Chem ... [more]

J. Thromb. Haemost. Nov. 01, 2003; 1(11);2360-7 [Pubmed: 14629470]

Throughput

  • Low Throughput

Curated By

  • BioGRID