An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

AP-1 recruitment to VAMP4 is modulated by phosphorylation-dependent binding of PACS-1.

Hinners I, Wendler F, Fei H, Thomas L, Thomas G, Tooze SA

The R-SNARE VAMP4, which contains a dileucine motif, binds to the AP-1 (adaptor protein-1) subunit mu 1a, but not mu 1b, or the GGAs (Golgi-associated gamma ear containing ARF binding proteins). Serine 20 and leucines 25,26 are essential for this binding. AP-1 association with VAMP4 is enhanced when serine 30, in an acidic cluster, is phosphorylated by casein kinase 2. ... [more]

EMBO Rep. Dec. 01, 2003; 4(12);1182-9 [Pubmed: 14608369]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VAMP4 AP1M1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hinners I (2003)	Low	-	BioGRID	-
VAMP4 AP1M1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hinners I (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

VAMP4

Gene Ontology Biological Process

Gene Ontology Molecular Function

SNAP receptor activity [IBA]SNARE binding [IBA]

Gene Ontology Cellular Component

AP1M1