Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Roles of TRP14, a thioredoxin-related protein in tumor necrosis factor-alpha signaling pathways.

Jeong W, Chang TS, Boja ES, Fales HM, Rhee SG

The possible roles of a 14-kDa human thioredoxin (Trx)-related protein (TRP14) in TNF-alpha signaling were studied in comparison with those of Trx1 by RNA interference in HeLa cells. Depletion of TRP14 augmented the TNF-alpha-induced phosphorylation and degradation of I kappa B alpha as well as the consequent activation of NF-kappa B to a greater extent than did Trx1 depletion. Deficiency ... [more]

J. Biol. Chem. Jan. 30, 2004; 279(5);3151-9 [Pubmed: 14607843]

Throughput

  • Low Throughput

Curated By

  • BioGRID