BAIT

FNBP1

FBP17
formin binding protein 1
GO Process (0)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

TNKS

ARTD5, PARP-5a, PARP5A, PARPL, TIN1, TINF1, TNKS1, pART5
tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The formin-binding protein 17, FBP17, binds via a TNKS binding motif to tankyrase, a protein involved in telomere maintenance.

Fuchs U, Rehkamp GF, Slany R, Follo M, Borkhardt A

In acute myelogenous and lymphoid leukemias, rearrangements involving the MLL (mixed lineage leukemia) gene at chromosome 11q23 are frequent. The truncated MLL protein is fused in-frame to a series of partner proteins. We previously identified the formin-binding protein 17 (FBP17) as such an MLL fusion partner. In this study, we explored in vivo physiological interaction partners of FBP17 using a ... [more]

FEBS Lett. Nov. 06, 2003; 554(1);10-6 [Pubmed: 14596906]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FNBP1 TNKS
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID